2019-nCoV Spike protein RBD (N501Y),  HRP conjugated (C19SD-G231D)

2019-nCoV Spike protein RBD (N501Y), HRP conjugated (C19SD-G231D)

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Description: Recombinant 2019-nCoV Spike protein S1 subunit, RBD (N501Y) (319-541) was expressed in CHO cells using a C-terminal his tag, and then conjugated to Horse peroxidase (HRP).

Species: SARS-CoV-2

Tag: HIS tag

Expression System: CHO Cells

Sequence: 319-541

Genbank Number: MN908947

 

Activity:  Binding ability measured in a functional ELISA. 2019-nCoV RBD (L452R, T478K) binds to immobilized human ACE2 (19-740) protein (A51C2-G341F).

Storage and Stability:  Store product at –20oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Gene Aliases : 2019-nCoV RBD, SARS-CoV-2 spike RBD, novel coronavirus spike RBD, nCoV spike RBD, Alpha

Scientific Background: The receptor binding domain (RBD) of the SARS-CoV-2 spike glycoprotein recognizes the host ACE2 receptor and is a major determinant of viral entry and neutralization (1). SARS-CoV-2 variant, B.1.1.7 lineage, reported from the United Kingdom possess mutation N501Y at one of the six major contact residues in the receptor-binding domain involved in ACE2 binding. MD studies indicate that the mutant N501Y might increase the stability of the RBD domain with the ACE2 protein thereby resulting in higher virulence compared to the wild type strain (2). As new variants displace the first-wave virus, it is pivotal to evaluate their transmissibility, virulence and their possible tendency to escape antibody neutralization (3).

References : 

  1. Lan J, et al: Crystal structure of the 2019-nCoV spike receptor-binding domain bound with the ACE2 receptor. bioRxiv. doi: https://doi.org/10.1101/2020.02.19.956235.
  2. Sharma T, et al: Unbuttoning the impact of N501Y mutant RBD on viral entry mechanism: A computational insight. bioRxiv 2020; doi: https://doi.org/10.1101/2020.12.30.424906
  3. Starr TN, et al: Molecular dynamic simulation reveals E484K mutation enhances spike RBD-ACE2 affinity and the combination of E484K, K417N and N501Y mutations (501Y.V2 variant) induces conformational change greater than N501Y mutant alone, potentially resulting in an escape mutant. Cell. 2020, 182(5):1295-1310.

Product Sheets (By Lot #) :

J4076-15