2019-nCoV Spike protein RBD,  HRP conjugated (C19SD-G241D)

2019-nCoV Spike protein RBD, HRP conjugated (C19SD-G241D)

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Description: Recombinant 2019-nCoV Spike protein S1 subunit, RBD (319-541) was expressed in CHO cells using a C-terminal his tag, and then conjugated to Horse peroxidase (HRP).

Species: SARS-CoV-2

Tag: HIS tag

Expression System: CHO Cells

Sequence: 319-541

Genbank Number: MN908947

 

Activity:  Binding ability measured in a functional ELISA. 2019-nCoV Spike protein RBD binds to immobilized human ACE2 (19-740) protein (A51C2-G341F).

Storage and Stability:  Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Molecular Weight: 39 kDa

Gene Aliases : 2019-nCoV RBD, SARS-CoV-2 spike RBD, novel coronavirus spike RBD, nCoV spike RBD.

Scientific Background: The severe acute respiratory syndrome related novel coronavirus SARS-CoV-2 has caused the pandemic of the respiratory diseases (COVID-19) around the world in 2020 (1). The spike glycoprotein (S) of coronavirus belongs to the type I transmembrane protein, which contains two subunits, S1 and S2 (2), which is also known to be the key component to bind with host cells through the interaction with angiotensin-converting enzyme 2 (ACE2) (3). A receptor binding domain (RBD) of S1 can recognize the cell surface receptor and the mutation of RBD could cause higher motility rate (3).

References : 

  1. Zhou P, et al: A pneumonia outbreak associated with a new coronavirus of probable bat origin. Nature. 2020, 579:270-89.
  2. Xiao X, et al: The SARS-CoV S glycoprotein. Cell Mol Life Sci. 2004, 61 (19-20): 2428-30.
  3. Lan J, et al: Crystal structure of the 2019-nCoV spike receptor-binding domain bound with the ACE2 receptor. bioRxiv. doi: https://doi.org/10.1101/2020.02.19.956235.


Product Sheets (By Lot #) :

J4076-9