Cyclin T2 Protein(C86-30BG)
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Description :Full-length recombinant human Cyclin T2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species :Human
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence :Full-length
Genbank Number :NM_001241
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability, and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Western Blot
Molecular Weight :~102 kDa
Gene Aliases :CCNT2; CYCT2
Scientific Background :Cyclin T2 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle which function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclin T2 and its kinase partner CDK9 were found to be subunits of the transcription elongation factor p-TEFb. The p-TEFb complex containing this cyclin was reported to interact with, and act as a negative regulator of human immunodeficiency virus type 1 (HIV-1) Tat protein(1). In mammalian cells, all 3 CDK9/cyclin T combinations strongly activated a CMV promoter(2).
References :
1. Yang, Z.et.al: The 7SK small nuclear RNA inhibits the CDK9/cyclin T1 kinase to control transcription. Nature 414: 317-322, 2001.
2. Peng, J.et.al: Identification of multiple cyclin subunits of human P-TEFb. Genes Dev. 12: 755-762, 1998.
Product Sheets (By Lot #) :
Research Areas :Cancer, Cell Cycle