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BAD Protein (B03-30G)

BAD Protein (B03-30G)

  • $215.00


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Description : Recombinant full-length human BAD was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Species : Human

Tag : GST tag

Expression System : Sf9 insect cells using baculovirus

Sequence : Full length

Genbank Number : NM_032989

Purity : Sample Purity Data. For specific information on a given lot, see related technical data sheet. 

Storage, Stability and Shipping : Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications : Western Blot

Molecular Weight : ~47 kDa

Gene Aliases : BBC2, BCL2L8

Scientific Background : BAD is a member of the BCL-2 family of proteins that are known to be regulators of programmed cell death. BAD is a pro-apoptotic protein that forms a heterodimer complex with BCL-xL and BCL-2 which reverses the prosurvival activity of these proteins (1). The proapoptotic activity of BAD is regulated through its phosphorylation and this inhibits the pro-apoptosis function of BAD. Protein kinases such as AKT, RAF and RSK1 can phosphorylate BAD and RSK1-induced phosphorylation of BAD at ser112 suppresses BAD-mediated apoptosis in neurons. BAD inhibits G(1) to S phase transition in MCF7 breast cancer cells and overexpression of BAD inhibits cell growth as well as cyclin D1 expression (2).

References : 
1. Yang E, et al: Bad, a heterodimeric partner for Bcl-X(L) and Bcl-2, displaces Bax and promotes cell death. Cell 80: 285-291, 1995.

2. Fernando R, et al: Breast cancer cell proliferation is inhibited by BAD: regulation of cyclin D1. J Biol Chem. 2007 Sep 28;282(39):28864-73.

Product Sheets: (by Lot #):

H320-1.pdf

Research Areas : Apoptosis/Autophagy, Cancer, Cellular Stress, Neurobiology,