LCMT2 Protein(L322-30G)
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Description :Recombinant full-length human LCMT2 protein was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species :Human
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence :Full length
Genbank Number :NM_014793
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability, and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Methyltransferase Assay
Molecular Weight :90 kDa
Gene Aliases :PPM2; TYW4
Scientific Background :LCMT2, also named tRNA wybutosine-synthesizing protein 4 (TYW4), is an S-adenosylmethionine (SAM)-dependent enzyme that involves in the final step of wybutosine biosynthesis pathway (1). Structural study revealed that TYW4 uses one catalytic site for both methylation and methoxycarbonylation process (2).
References :
1. Noma, A., et al. Biosynthesis of wybutosine, a hyper-modified nucleoside in eukaryotic phenylalanine tRNA. EMBO J. 25(10):2142-54, 2006.
2. Suzuki, Y., et al. Structural basis of tRNA modification with CO2 fixation and methylation by wybutosine synthesizing enzyme TYW4. Nucleic Acids Res. 37(9):2910-25, 2009.
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Research Areas :Cell Cycle, Metabolic Disorder, Neurobiology