HOLIDAY SHIPPING UPDATE: All delivery service requests will not be processed on Monday, August 3, 2020 as it is British Columbia Day holiday. Regular delivery schedule will resume the next business day.
PAD1 Inhibitor Screening Kit (P310G-863)

PAD1 Inhibitor Screening Kit (P310G-863)

  • $690.00


FOR BULK ORDER REQUESTS PLEASE CONTACT US

Overview :The PAD1 Inhibitor Screening Kit is a direct Enzyme-Linked ImmunoSorbent Assay (ELISA) kit for use in PAD1-targeted inhibitor profiling assays. The kit includes recombinant full-length human PAD1 expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag (accession number NM_013358).

Components :

Part # Part Name Size Storage
AB99-61DM Detection Antibody, 500X 25 ul/1 Store kit box and contents at 4oC.
CP01-506 NeutrAvidin Coated Plate 1 plate/1
CS01-506 TMB Substrate 6 ml/1
P312-09 PAD Buffer 8 ml/1
SS01-09 Stop Solution 3 ml/1
T01-09 Trypsin Digestion Buffer 15 ml/1
WB20-09 Wash Buffer, 20X 15 ml/2
P312-37C PAD Cocktail, Active 5 ug/1 Store product at –20oC or –70oC as specified on individual component labels. For PAD enzymes, avoid repeated handling and multiple freeze/thaw cycles.
D86-09 DTT Solution 10 ul/1
T575-31N Trypsin, Active 200 ug/1
P312-58 PAD Substrate .5 mg/1
P312-310G PAD1, Active 50 ug/1


Representative Data :Representative Data Demonstration of high sensitivity and specificity of the assay achieved by using human recombinant PAD enzymes with PAD Activity Kit.

Assay Work Flow : Click here to see image

Technical Manual:

CoA Sheet :

SDS :

References :
1. Rothnagel, J.A., & Rogers, G.E. (1984). Citrulline in proteins from the enzymatic deimination of arginine residues. Methods Enzymol. 107, 624-31.

2.Raijmakers, R. et al. (2007). Methylation of Arginine Residues Interferes with Citrullination by Peptidylarginine Deiminases in vitro. J. Mol. Biol. 367, 1118–29.

3. Tilvawala, R., & Thompson, P.R. (2019). Peptidyl arginine deiminases: detection and functional analysis of protein citrullination. Curr. Opin. Struct. Biol. 20, 1-11.

4. Wildeman E., & Pires M.M. (2013). Facile fluorescence-based detection of PAD4-mediated citrullination. Chembiochem. 14, 963-7.

5. Clancy K.W. et al. (2016). Detection and identification of protein citrullination in complex biological systems. Curr. Opin. Chem. Biol. 30, 1-6.

Related Products :

Catalog Name Catalog #
PAD1, Active P312-310G
PAD2, Active P312-310BG
PAD2, Active P312-318BG
PAD3, Active P312-310CG
PAD4, Active P312-310DG
PAD Cocktail, Active P312-37C
PAD1 Inhibitor Screening Kit P310G-863
PAD Cocktail Inhibitor Screening Kit P37C-863
PAD2 (human) Inhibitor Screening Kit P318BG-863
PAD2 (mouse) Inhibitor Screening Kit P312-310BG
PAD3 Inhibitor Screening Kit P310CG-863
PAD4 Inhibitor Screening Kit P310DG-863
PAD Detection Kit P312-863