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PAD Cocktail Inhibitor Screening Kit (P37C-863)

PAD Cocktail Inhibitor Screening Kit (P37C-863)

  • $690.00


Overview :The PAD Cocktail Inhibitor Screening Kit is a direct Enzyme-Linked ImmunoSorbent Assay (ELISA) kit for use in PAD-targeted inhibitor profiling assays. The kit includes PAD cocktail, a mixture of 5 full-length PAD enzymes (1, 2, 3, 4, and 6) expressed by baculovirus in Sf9 insect cells using N-terminal GST tags (accession number NM_013358).

Components :

Part # Part Name Size Storage
AB99-61DM Detection Antibody, 500X 25 ul/1 Store kit box and contents at 4oC.
CP01-506 NeutrAvidin Coated Plate 1 plate/1
CS01-506 TMB Substrate 6 ml/1
P312-09 PAD Buffer 8 ml/1
SS01-09 Stop Solution 3 ml/1
T01-09 Trypsin Digestion Buffer 15 ml/1
WB20-09 Wash Buffer, 20X 15 ml/2
P312-37C PAD Cocktail, Active 5 ug/1 Store product at –20oC or –70oC as specified on individual component labels. For PAD enzymes, avoid repeated handling and multiple freeze/thaw cycles.
D86-09 DTT Solution 10 ul/1
T575-31N Trypsin, Active 200 ug/1
P312-58 PAD Substrate .5 mg/1
P312-37C PAD Cocktail, Active 50 ug/1

Representative Data :Representative Data Demonstration of high sensitivity and specificity of the assay achieved by using human recombinant PAD enzymes with PAD Activity Kit.

Assay Work Flow : Click here to see image

Technical Manual:

CoA Sheet :


References :
1. Rothnagel, J.A., & Rogers, G.E. (1984). Citrulline in proteins from the enzymatic deimination of arginine residues. Methods Enzymol. 107, 624-31.

2.Raijmakers, R. et al. (2007). Methylation of Arginine Residues Interferes with Citrullination by Peptidylarginine Deiminases in vitro. J. Mol. Biol. 367, 1118–29.

3. Tilvawala, R., & Thompson, P.R. (2019). Peptidyl arginine deiminases: detection and functional analysis of protein citrullination. Curr. Opin. Struct. Biol. 20, 1-11.

4. Wildeman E., & Pires M.M. (2013). Facile fluorescence-based detection of PAD4-mediated citrullination. Chembiochem. 14, 963-7.

5. Clancy K.W. et al. (2016). Detection and identification of protein citrullination in complex biological systems. Curr. Opin. Chem. Biol. 30, 1-6.

Related Products :

Catalog Name Catalog #
PAD1, Active P312-310G
PAD2, Active P312-310BG
PAD2, Active P312-318BG
PAD3, Active P312-310CG
PAD4, Active P312-310DG
PAD Cocktail, Active P312-37C
PAD1 Inhibitor Screening Kit P310G-863
PAD2 (mouse) Inhibitor Screening Kit P310BG-863
PAD2 (human) Inhibitor Screening Kit P318BG-863
PAD3 Inhibitor Screening Kit P310CG-863
PAD4 Inhibitor Screening Kit P310DG-863
PAD Detection Kit P312-863