PAD Detection Kit (P312-863)
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Overview :The PAD Activity Kit is a direct Enzyme-Linked ImmunoSorbent Assay (ELISA) kit for the quantitative determination of total PAD activity in cell or tissue lysates. The kit includes a positive control PAD Cocktail, including five full length recombined PAD proteins (1, 2, 3, 4 and 6) expressed by baculovirus in Sf9 insect cells using N-terminal GST tags
Components :
| Part # | Part Name | Size | Storage |
| AB99-61DM | Detection Antibody, 500X | 25 ul/1 | Store kit box and contents at 4oC. |
| CP01-506 | NeutrAvidin Coated Plate | 1 plate/1 | |
| CS01-506 | TMB Substrate | 6 ml/1 | |
| P312-09 | PAD Buffer | 8 ml/1 | |
| SS01-09 | Stop Solution | 3 ml/1 | |
| T01-09 | Trypsin Digestion Buffer | 15 ml/1 | |
| WB20-09 | Wash Buffer, 20X | 15 ml/2 |
| P312-37C | PAD Cocktail, Active | 5 ug/1 | Store product at –20oC or –70oC as specified on individual component labels. For PAD enzymes, avoid repeated handling and multiple freeze/thaw cycles. |
| D86-09 | DTT Solution | 10 ul/1 | |
| T575-31N | Trypsin, Active | 200 ug/1 | |
| P312-58 | PAD Substrate | .5 mg/1 |
Representative Data :Representative Data Demonstration of high sensitivity and specificity of the assay achieved by using human recombinant PAD enzymes with PAD Activity Kit.
Assay Work Flow : Click here to see image
Technical Manual:
CoA Sheet :
SDS :
References :
1. Rothnagel, J.A., & Rogers, G.E. (1984). Citrulline in proteins from the enzymatic deimination of arginine residues. Methods Enzymol. 107, 624-31.
2.Raijmakers, R. et al. (2007). Methylation of Arginine Residues Interferes with Citrullination by Peptidylarginine Deiminases in vitro. J. Mol. Biol. 367, 1118–29.
3. Tilvawala, R., & Thompson, P.R. (2019). Peptidyl arginine deiminases: detection and functional analysis of protein citrullination. Curr. Opin. Struct. Biol. 20, 1-11.
4. Wildeman E., & Pires M.M. (2013). Facile fluorescence-based detection of PAD4-mediated citrullination. Chembiochem. 14, 963-7.
5. Clancy K.W. et al. (2016). Detection and identification of protein citrullination in complex biological systems. Curr. Opin. Chem. Biol. 30, 1-6.
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