VLK Protein(V05-31G)
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Description :Recombinant human VLK (129-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species :Human
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence :129-end
Genbank Number :NM_138370
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Western Blot
Molecular Weight :~69 kDa
Gene Aliases :SGK493; PKDCC
Scientific Background :VLK or vertebrate lonesome kinase is a protein kinase which is first expressed in E-cadherin-positive anterior visceral endoderm and mesendoderm, and later its expression is confined to E-cadherin-negative mesenchyme. VLK regulates the rate of protein export from the Golgi and thereby plays an important role in the formation of functional stroma by mesenchymal cells (1). Targeted disruption of VLK leads to a defect in lung development and delayed ossification of endochondral bone. VLK deficient mice display neonatal lethality due to respiratory failure, with a suckling defect arising from a cleft palate. VLK is required for the appropriate timing of flat proliferative chondrocyte differentiation (2).
References :
1. Kinoshita. et.al: The novel protein kinase Vlk is essential for stromal function of mesenchymal cells.Development 136: 2069-2079, 2009.
2. Imuta, Y. et al: Short limbs, cleft palate, and delayed formation of flat proliferative chondrocytes in mice with targeted disruption of a putative protein kinase gene, Pkdcc (AW548124). Dev Dyn. 2009 Jan;238(1):210-22.
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Research Areas :Cancer, Cytoplasmic Tyrosine Kinases, Invasion/Metastasis, Neurobiology, Ser/Thr Kinases, Cancer, Neurobiology, Invasion/Metastasis, Ser/Thr Kinases, Cytoplasmic Tyrosine Kinases